Phred Quality Score Analysis

Use Amplicon Express'Phred Quality Score Analysis tool to develop useful statistics regarding the quality of your sequence data as generated by the Phred analysis program. Results will be displayed in a printer friendly format.

Don't forget:

• It is best to trim vector before running this analysis
• A valid FASTA formatted sequence starts with a header line:

  >This is a valid header line

• Only 'agctAGCTxXnN' are allowed in sequence data (header lines are excluded from this rule)

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Other Tools: Sequence Finder |  Protein Translation Tool |  Oligo Tm Calculator | 
Phred Quality Score Analyzer  | Vector Trimming Tool

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Sample Data

• Cut and paste the sample data to see what your results will look like

>PGEM.M13F 108 0 108 ABI trimmed
15 15 20 22 19 22 23 29 29 35 25 26 15 18 18 46 39 42 42 51 51 51 44 37 32 26 26 32 29 29 29 29 29 35 35 37 46 42 42 42 51 45 45 39 39 39 40 40 37 56 51 51 51 51 45 45 51 45 45 45 51 51 51 56 56 51 51 51 51 51 56 45 45 45 45 45 45 51 56 56 56 56 56 56 56 56 56 51 51 51 51 40 45 45 40 40 45 51 56 56 56 56