Metagenomic
Understanding all the organisms in a Metagenomic sample is very important for many areas of research (bioremediation, extremophile studies, biodiversity, pollution resistance and other important areas of research).Having a genomic DNA library from a Metagenomic Sample can provide a wealth of information and years of research possibilities. However, many of these samples are composed of a wide variety of genomes making it overwhelming and difficult to get started.
Amplicon Express scientists have made this task easier with a TWO STAGE STRATEGY:
First: we will extract DNA from your metagenomic sample and prepare either a large insert Fosmid library or BAC library. This resource will allow in-depth study of particular gene regions contained from organisms present in the sample.
Second: we perform PCR amplification on a diagnostic region of your choice (such as 16S ribosomal subunit) and construction of a TA cloned small insert library of this amplified DNA. This library provides the data needed for a basic understanding of WHAT types of organisms are present in the sample. We sequence 96 clones of the PCR amplified library.
With these TA Library sequence data, probes can be designed to screen your large insert Fosmid or BAC library arrayed on nylon filters.
This strategy can be employed multiple times on the same libraries using different starting amplification primers and gene specific probes for screening the genomic DNA library resource.
Amplicon scientists have worked on many important and interesting metagenomic projects allowing us to refined this strategy: soil samples from river beds (i.e. soil collected above and below a hydroelectric facility and paper mill), natural hot spring bacterial mats, and filtered sea water for biodiversity studies, to name a few.
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