Instructions for Snap Freezing Starting Materials
- For animal tissue:
- Collect the recommended amount of desired tissue. For more information please see the guidelines for collection of starting materials page.
- Place the tissue into a 50ml plastic screw cap centrifuge tube.
- Label the tube with the following information:
- Organism name
- Date collected
- Project Quote Number
Fill a mortar or similar receptacle with liquid nitrogen. Allow it to cool the vessel.
Once the mortar is adequately chilled, place centrifuge tube into the liquid nitrogen.
Allow the contents of the tube to freeze-this may take a few minutes. Add more liquid nitrogen as needed.
Once the material is frozen, place it in a plastic bag and store at -80c until shipment.
For instructions on how to ship starting materials, please click here.
- For Plant Tissue:
- Collect the recommended amount of desired tissue. For more information please see the guidelines for collection of starting materials page.
- Wrap the tissue inside aluminum foil. It is helpful to separate the tissue into 2 or 3 smaller packages.
- Label the packages with the following information:
- Organism name
- Date collected
- Project Quote Number
Fill a mortar or similar receptacle with liquid nitrogen. Allow it to cool the vessel.
Once the mortar is adequately chilled, place the foil package into the liquid nitrogen.
Allow the package to freeze-this may take a few minutes. Add more liquid nitrogen as needed.
Once the material is frozen, place it in a plastic bag and store at -80c until shipment.
for more information on how to snap freeze materials, please contact Robert Bogden, bogden@genomex.com.