Commonly Used and Universal Primers
Below are some commonly used universal primers and their corresponding sequences. Amplicon Express supplies all universal primers listed below free of charge when you are sequencing your plasmid or PCR based samples with us.
| Primer Name | Sequence (5' to 3') | Tm |
|---|---|---|
| >21m13.f | TGTAAAACGACGGCCAGT | 54C |
| >21m13.r | CAGGAAACAGCTATGACC | 54C |
| >pTrCHis.For | GAGGTATATATTAATGTATCG | 54C |
| >pTrCHis.Rev | GATTTAATCTGTATCAGG | 48C |
| >puc18.For | CCAGTCACGACGTTGTAA | 54C |
| >puc18.Rev | CGGATAACAATTTCACACA | 52C |
| >sp6 | ATTTAGGTGACACTATAG | 48C |
| >t3 | ATTAACCCTCACTAAAGGGA | 56C |
| >t7 | TAATACGACTCACTATAGGG | 56C |
| >t7_terminator | GCTAGTTATTGCTCAGCGG | 58C |
| >TrxFus | TTCCTCGACGCTAACCTG | 56C |
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