Nylon Filters

Screening large numbers of clones can be facilitated by robotically printing viable, bacterial cells from 96 or 384 well plates onto nylon filters. After overnight growth of the clones, the filters are processed and ultimately, the DNA from each clone is permanently fixed to a known position on the filter. These clones can be screened using standard DNA hybridization procedures. Positive clones are identified and can be retrieved from the original plates. Typically, we print 22 cm x 22 cm filters at two densities: 9,216 clones/filter or 18,432 clones/filter. All clones are printed in duplicate and we can customize the filters to meet your demands.

Related Nylon Filter Services:

Request A Quote for Nylon Filters

Please email Robert Bogden the following details regarding your Nylon Filter project, or request an online quote:

Desired filter size and density? Please select one:

Filter Size	Density	# of Clones	In Duplicate?
8 cm X 12 cm	Single-density	3072	Yes
8 cm X 12 cm	Double-density	6144	Yes
22.5cm X 22.5cm	Single-density	18432	Yes
22.5cm X 22.5cm	Double-density	36864	Yes

Library Type: BAC, fosmid, cosmid, cDNA, or shotgun?
Library Details: antibiotic resistance, vector, special growth medium, inducible copy control requirements?
Would you like positive controls placed on the filters? If so, please indicate whether you will be supplying the positive control as a glycerol stock or agar stab. Also indicate the antibiotic resistance, vector, special growth medium, and inducible copy control requirements of your control.
Please indicate whether the library is stored in 96 or 384-well plates.
Please indicate the order in which you would like your plates gridded if the library plates are not in sequential order.

Packaging Recommendations: 96 or 384-well Plates:

The following list is a set of recommendations for packaging your 96 or 384-well plates for shipment to our lab:

Stack plates on top of each other and secure them with rubber bands
Wrapped secured plates with bubble wrap or packing paper, or place in a box to protect plates from damage by loose dry ice pieces.
Place boxed (or wrapped) plates into a styrofoam container and pack the container tightly with dry ice. Note that dry ice will sublimate during shipping, so it is critical to separate your plates from the dry ice, as loose plates will get damaged during shipment.
Amplicon Express does not recommend use of adhesive plate seals on your plates due to the high likelihood of cross contamination when the thawed plates are opened for processing. Plates that are frozen via dry ice do not need to be sealed, though using a plastic plate cover, and stacking/rubber-banding the plates as noted above is important to your plates arriving in good condition in our laboratory.

Please contact Keith Stormo for more detailed instructions regarding the sending of your materials to our lab.

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