BAC-MAPS: Pooling for Very Large Genomes

The BACMAPS (BAC Minimally Amplified Pooling Strategy) system enables researchers to use PCR for screening a BAC library constructed from an organism with a very large genome, typically larger than 15 Gb. Since the expense of picking millions of clones may be cost prohibitive, we have developed a system allowing screening of millions of clones with a few (48-384) PCR experiments and then following those results (a 'hit') to another plate and screening that second plate DNA by a another round of 96 PCR experiments. This second round PCR identifies a well that contains your BAC clone of interest as well as other BAC clones. The exact plate and well position containing your BAC clone of interest, together with 25-35 other BAC clones (or up to 80 in special cases) can be correctly identified in 192 PCR experiments. Following identification of the specific plate and well position, the specific well is thawed, mixed, and plated on selective media for picking of individual clones.

BAC-MAPS Services

• Request A Quote
• BAC-MAPS User's Guide

BAC-MAPS Quote by Email

If your organism has a haploid genome size larger than 10,000 Mbp (10 Gb) and funding is a key concern, we recommend our BAC MAPS Pooling System. Cost savings is tremendous at the expense of taking slightly longer to identify a positive clone of interest. Please send the following information to Robert Bogden.

1. Name of organism and type of starting material (tissue, cells, genomic DNA embedded in agar plugs)?
2. Estimated haploid genome size?
3. Amount of cloned coverage needed (if known)?
4. Preferred cloning enzyme (HindIII, MboI, BamHI or EcoRI)? (Note: We recommend HindIII)
5. Estimated date to start project?
6. Any related products needed (i.e. a BAC library, nylon filters, copies, or BAC fingerprinting/mapping)?
7. Complete contact information (address, telephone, fax)?

With this information, we will generate a quote for building a BAC clone pooling system with the following options of cloned coverage: 15,000 Mbp, 30,000 Mbp, 60,000 Mbp or 90,000 Mbp. Greater levels of cloned coverage are available; please let us know the amount needed, or we can recommend coverage based on the requirements of your project.

BAC-MAPS Online Quote Request

Request A BAC-MAPS Quote