Latest Publications
A first generation BAC-based physical map of the rainbow trout genome 2009-10-08
Background: Rainbow trout (Oncorhynchus mykiss) are the most-widely cultivated cold freshwater fish in
the world and an important model species for many research areas. Coupling great interest in this species
as a research model with the need for genetic improvement of aquaculture production efficiency traits
justifies the continued development of genomics research resources. Many quantitative trait loci (QTL)
have been identified for production and life-history traits in rainbow trout. A bacterial artificial
chromosome (BAC) physical map is needed to facilitate fine mapping of QTL and the selection of positional
candidate genes for incorporation in marker-assisted selection (MAS) for improving rainbow trout
aquaculture production. This resource will also facilitate efforts to obtain and assemble a whole-genome
reference sequence for this species. |
A sequence-based survey of the complex structural organization of tumor genomes 2008-03-25
Background: The genomes of many epithelial tumors exhibit extensive chromosomal
rearrangements. All classes of genome rearrangements can be identified using end sequencing
profiling, which relies on paired-end sequencing of cloned tumor genomes.
|
A Strong Candidate for the Breast and Ovarian Cancer Susceptibility Gene BRCA1 1994-10-07
A strong candidate for the 17q-linked BRCA1 gene, which influences susceptibility to
breast and ovarian cancer, has been identified by positional cloning methods. Probable
predisposing mutations have been detected in five of eight kindreds presumed to segregate
BRCA1 susceptibility alleles. The mutations include an 11-base pair deletion, a
1-base pair insertion, a stop codon, a missense substitution, and an inferred regulatory
mutation. The BRCA 1 gene is expressed in numerous tissues, including breast and ovary,
and encodes a predicted protein of 1863 amino acids. This protein contains a zinc finger
domain in its amino-terminal region, but is otherwise unrelated to previously described
proteins. Identification of BRCA1 should facilitate early diagnosis of breast and ovarian
cancer susceptibility in some individuals as well as a better understanding of breast
cancer biology. |
One large-insert plant-transformation-competent BIBAC library and three BAC libraries of Japonica rice for genome research in rice and other grasses 2002-08-22
We report one large-insert BIBAC library and three BAC libraries for japonica rice cv Nipponbare. The BIBAC library was constructed in the HindIII site
of a plant-transformation-competent binary vector (pCLD04541) and the three BAC libraries were constructed in the BamHI, HindIII and EcoRI sites of a
BAC vector (pECBAC1), respectively. Each library contains 23,040 clones, has an average insert size of 130 kb, 170 kb, 150 kb and 156 kb, and covers 6.7x,
8.7x, 7.7x and 8.0 x rice haploid genomes, respectively. The combined libraries contain 92,160 clones in total, covering 31.1 x rice haploid genomes. To demonstrate
their utility, we screened the libraries with 55 DNA markers mapped to chromosome 8 of the rice genetic maps and analyzed a number of clones by the restriction
fingerprinting and contig assembly method. The results indicate that the libraries completely cover the rice genome and, thus, are well-suited for genome research in
rice and other gramineous crops. The BIBAC library represents the first plant-transformation-competent large-insert DNA library for rice, which will streamline
map-based cloning, functional analysis of the rice genome sequence and molecular breeding in rice and other grass species. These libraries are being used
in the development of a whole-genome, BAC/BIBAC-based, integrated physical, genetic and sequence map of rice and in the research of genome-wide
comparative genomics of grass species. |