Below are some commonly used universal primers and their corresponding sequences. Amplicon Express
supplies all universal primers listed below free of charge when you are sequencing your plasmid or PCR based
samples with us.
| Primer Name | Sequence (5' to 3') | Tm |
| >21m13.f | TGTAAAACGACGGCCAGT | 54C |
| >21m13.r | CAGGAAACAGCTATGACC | 54C |
| >pTrCHis.For | GAGGTATATATTAATGTATCG | 54C |
| >pTrCHis.Rev | GATTTAATCTGTATCAGG | 48C |
| >puc18.For | CCAGTCACGACGTTGTAA | 54C |
| >puc18.Rev | CGGATAACAATTTCACACA | 52C |
| >sp6 | ATTTAGGTGACACTATAG | 48C |
| >t3 | ATTAACCCTCACTAAAGGGA | 56C |
| >t7 | TAATACGACTCACTATAGGG | 56C |
| >t7_terminator | GCTAGTTATTGCTCAGCGG | 58C |
| >TrxFus | TTCCTCGACGCTAACCTG | 56C |
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